Fig. 3
Expression analysis of FAT1 in Colo205 cells. a Western blot analysis of total Colo205 extracts. Colo205 were lysed, separated on SDS-PAGE (25 micrograms/lane, corresponding to approximately 105 cells), transferred to nitrocellulose membranes and incubated with mAb198.3. Molecular weight standards (MW) are reported on the left side. b-c Confocal microscopy analysis: Cells were grown on glass coverslips, fixed and and incubated with the mAb198.3. Cells were stained with Alexafluor 488-labeled goat anti-mouse antibodies to detect FAT1 (green) and DAPI to visualize nuclei (blue). Flow cytometry analysis showing that both free mAb198.3_Cy5 d and AuCOOH(Cy5)_ mAb198.3 could bind Colo205 cells while the MFI of AuCOOH(Cy5)_ mAb198.3 is much higher than that of free mAb198.3_Cy5 e