Fig. 1

UA inhibited the growth and induced apoptosis of NSCLC cells in the dose-and time-dependent fashion. a, H1299 cells were treated with increased concentrations of UA for up to 72 h. b, NSCLC cell lines indicated were treated with UA (30 μM) for 48 h. The cell viability was determined using the MTT assay as described in the Materials and Methods Section and was expressed as percentage of control in the mean ± SD of three separate experiments. *Indicates significant difference as compared to the untreated control group (P < 0.05). c, H1299 cells were treated with increased concentrations of UA for 24 h. Afterwards, cells were harvested for analysis of apoptosis using the Annexin V-FITC/PI Apoptosis Detection Kit as detailed in Materials and Methods Section. The AB3 quardrant (annexin V-/PI-), AB4 quadrant (annexin V+/PI-) and AB2 quadrant (annexin V+/PI+) of the histograms indicated the percentage of non-apoptotic cells, early apoptosis and late apoptosis, respectively. Data are expressed as a percentage of total cells. Values in bar graphs were given as the mean ± SD from three independent experiments performed in triplicate. *indicates significant difference as compared to the untreated control group (P < 0.05). d, Caspase 3/7 activity assay was performed on H1299 cells treated with or without UA (30 μM) for 48 h. Relative caspase 3/7 activity is indicated as percentage of untreated control cells. Results represent those obtained in three experiments. *Indicates significant difference as compared to the untreated control group (P < 0.05)