Fig. 1

Relative expression of Loc554202 in colorectal cancer tissues and cells compared with adjacent normal tissues and normal colonic epithelial cells. a The relative expression of Loc554202 in colorectal cancer tissues (n = 48) compared with corresponding non-tumor tissues (n = 48). The Loc554202 expression was examined by qRT-PCR and was normalized to the GAPDH expression. The results are presented as the fold-change in tumor tissues relative to normal tissues (shown as -ΔΔCT). b and c) The data are presented as the relative expression levels in tumor tissues. The Loc554202 expression was significantly lower in patients with a higher pathological stage and larger tumors. d The Loc554202 expression was assessed by qRT-PCR in colorectal cancer cell lines (HT-29, RKO, SW480, HCT116, DLD1) and was compared with the normal human colonic epithelial cell line (HCoEpiC). e The relative expression levels of Loc554202 following the treatment of HCT116 and DLD1 cells with pCDNA-Loc554202 and empty vector. (f and g) The level of Loc554202 expression in HCT116 and DLD1 cells following treatment with 5-aza-dC (0, 5, 10 μM).* P < 0.05 and ** P < 0.01