Fig. 6

DDR2 induces EMT and up-regulates the expression of MTI-MMP and MMP2 through ERK2/SNAIL1 signaling in HCC cells. a Hep3B cells that had been transfected with DDR2 expressing plasmid or control plasmid, respectively, were subjected to western blotting for ERK1/2, p-ERK1/2, SNAIL1, Vimentin and E-cadherin. b MHCC-97H cells that had been transfected with control-shRNA, DDR2-shRNA, control-siRNA, ERK2-siRNA, control-siRNA or SNAIL1-siRNA, respectively, were subjected to western blotting for DDR2, ERK1/2, p-ERK1/2, SNAIL1, Vimentin and E-cadherin). c Co-immunoprecipitation assays showed that ERK2 was bound with SNAIL1 directly. d Hep3B cells that had been transfected with DDR2 expressing plasmid or control plasmid, respectively, were subjected to western blotting for ERK1/2, p-ERK1/2, SNAIL1, MTI-MMP and MMP2. e MHCC-97H cells that had been transfected with control-shRNA, DDR2-shRNA, control-siRNA, ERK2-siRNA, control-siRNA or SNAIL1-siRNA, respectively, were subjected to western blotting for DDR2, ERK1/2, p-ERK1/2, SNAIL1, MTI-MMP and MMP2. f HCC cells that had been transfected with indicated plasmids were added to plates coated with collagen I (2 mg/ml, BD Biosciences) for 8 h and western blotting were performed