Fig. 5

PARP cleavage induced by cobimetinib. Neuroblastoma cell lines IMR-32, SHEP and IMR-5 were incubated in the absence or presence of cobimetinib (1 μM) for 24 hour and cell lysates were analyzed by western blotting using antibodies specific for PARP and its cleaved fragment. Actin was used as loading control. Data presented is representative of two separate experiments