Fig. 6

The flow cytometric analysis of apoptosis in NB cells using FITC-annexin V and PI double staining. (a) Apoptosis was evaluated after treating NB cells with 1 μM of cobimetinib for 24 hour, and staining with Annexin-V. Annexin V+/PI− (lower right quadrant) areas represent early apoptotic cells, and Annexin V+/PI+ (upper right quadrant) areas for late apoptotic or necrotic cells. (b). Quantitative analysis of the percentage of early apoptotic cells in all NB cell lines. Data presented is representative of two separate experiments