Fig. 7

Time course analysis of cobimetinib on MAPK cascade pathway: Cells from three NB cell lines (IMR-32 (a), IMR-5 (b) and SHEP (c)) were harvested after treatment with 1 μM cobimetinib or DMSO control for the indicated periods. Cells were then lysed in protease and phosphatase containing buffer and probed for total and phosphorylated MEK by western blot analysis. Data presented is representative of three separate experiments