Fig. 2

Increased levels of E-selectin detected on the surface of human cerebral microvascular endothelial cells in response to lung tumour CM and secreted-proteins. Confluent hCMEC/D3 cells were stimulated for 4 h with A549 and SK-MES-1 CM (a) along with CC (c), CL (d), IGFBP-7 (e), VEGF (f) and TNF-α (g). E-selectin levels from 3 separate experiments, carried out in quadruplets, were statistically analysed (n ≥ 12, mean ± SD, *p ≤ 0.05 and **p ≤ 0.01, ***p ≤ 0.005 and ****p ≤ 0.0001). Enhanced E-selectin was also detected within 30 min of CM (a) and secreted-proteins (b) exposure (*p ≤ 0.05 and **p ≤ 0.01, n = 6)