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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: Loss of the endothelial glycocalyx is associated with increased E-selectin mediated adhesion of lung tumour cells to the brain microvascular endothelium

Fig. 4

E-selectin inhibition attenuated adhesion of A549 and SKMES-1 lung cancer cells to human cerebral microvascular endothelial cells. Confluent hCMEC/D3 cells were treated with A549 CM (a), SK-MES-1 CM (b) and secreted-proteins for 24 h prior to exposing the relevant channels with anti-E-selectin antibody for 30mins. The bars represent cancer cell adhesion to brain endothelial monolayer calculated as a percentage of control obtained on 3 separate occasions. (* p ≤ 0.05 vs control, #p ≤ 0.05, ##p ≤ 0.01 vs anti-E-selectin). c FC analysis of adhesion molecule ligands and receptors, CD49d, CD11a, CD11b, PSGL-1 and sLeX, on lung tumour cells. The presented data was collected from 3 different experiments

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