Fig. 2

Cell membrane localization of CD98 is mediated by basigin in HCC cells. a Changes in the molecular expression patterns were detected in control and SMMC-7721shCD98 cells. Control or SMMC-7721shCD98 cell lysates (30 μg) were subjected to western blot to analyze the expression of basigin, β1-integrin-A, β1-integrin, p-Akt, Akt and α-tubulin (reference control). b The membrane expression of β1-integrin-A and β1-integrin were analyzed using flow cytometry after overexpression of basigin or CD98, respectively. Black histograms (Blank) represent staining with isotype control. c The membrane localization of CD98 after basigin was knockdown or overexpressed. Mean fluorescence intensities (MFI) of each group was indicated beside the histograms. d MFI ratio of transfected cells and control cells. The results shown are representative of three independent experiments. ** P < 0.01. eThe mRNA and protein expression of CD98 in SMMC7721 cells with basigin knockdown or overexpression. After SMMC-7721 cells were transfected with basigin siRNA or pcDNA-3.1-CD147 for 36 h, the mRNA levels of basigin and CD98 were analyzed by RT-PCR. The results shown are representative of three independent experiments. ** P < 0.01 (f) Analysis of the total protein levels of basigin and CD98 by western blotting. The samples were first transfected as described in part (E) and then subjected to western blotting