Fig. 1

ALK and MET expression in RMS cell lines and tumour samples. a RT-PCR analysis showing the expression of ALK and MET mRNAs in RH4 and RH30, two ARMS cell lines, but faintly in RD and RD18, two ERMS cell lines. GAPDH expression used as the internal control. b Western blotting experiments showing higher levels of both total ALK and MET proteins in ARMS than in ERMS cells. The activated forms of both ALK and MET proteins were observed in ARMS samples using the specific p-ALK (Tyr1604) and p-MET (Tyr1234/Tyr1235) antibodies, respectively. Tubulin expression was used as the internal control. c Expression of ALK and MET mRNAs measured by RT-PCR in a panel of ARMS and ERMS tumour samples