Fig. 3

lncTCF7 is transcriptionally regulated by STAT3 in response to IL-6 stimulation. a Schematic representation of lncTCF7 promoter with seven potential SIEs and the primer pair used in ChIP-PCR assays. The reporter construct lncTCF7-Luc and its truncated and mutated derivatives are also shown. b Transcription activity in response to IL-6 treatmemt for 6 h measured by luciferase assay in SK-Hep-1 cells with a series of deletion mutants of lncTCF7-luc (internal control, pRL-TK). *p < 0.05. c Relative luciferase activity 6 h after IL-6 incubation in SK-Hep-1 cells transfected with the wild-type or SIE mutated lncTCF7 promoter reporter construct. *p < 0.05. d Chromatin prepared from SK-Hep-1 cells stimulated with IL-6 for 1 h was immunoprecipitated with the indicated antibodies; PCR was performed on immunoprecipitated DNAs or soluble chromatin using specific primer pair for the lncTCF7 promoter