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Table 1 Primer and siRNA list

From: Long noncoding RNA lncTCF7, induced by IL-6/STAT3 transactivation, promotes hepatocellular carcinoma aggressiveness through epithelial-mesenchymal transition

mRNA/gene promoter

sequence (5′-3′)

Experimental use

linc00974

5′-TCTAACGTGCCTGGGACCTA-3′(forward)

Real-time PCR

 

5′-AAATGCCTACCGCCAGTTCA-3′(reverse)

 

HOTAIR

5′-CAGTGGGGAACTCTGACTCG-3′(forward)

Real-time PCR

 

5′-GTGCCTGGTGCTCTCTTACC-3′(reverse)

 

H19

5′-ACTCAGGAATCGGCTCTGGAA-3′(forward)

Real-time PCR

 

5′-CTGCTGTTCCGATGGTGTCTT-3′(reverse)

 

DANCR

5′-GCGCCACTATGTAGCGGGTT-3′(forward)

Real-time PCR

 

5′-TCAATGGCTTGTGCCTGTAGTT-3′(reverse)

 

lncTCF7

5′-AGGAGTCCTTGGACCTGAGC-3′(forward)

Real-time PCR

 

5′-AGTGGCTGGCATATAACCAACA-3′(reverse)

 

Dreh

5′-CCTGTATGACGATGGAGCCT-3′(forward)

Real-time PCR

 

5′-TGACACATTTGCGATGGGTAT-3′(reverse)

 

lncRNA MVIH

5′-GAGACAGGATTTAGCCGTGTTG-3′(forward)

Real-time PCR

 

5′-AGCACTTTGGAAGGCTTAGACA-3′(reverse)

 

lncRNA-HEIH

5′-CCTCTTGTGCCCCTTTCTT-3′(forward)

Real-time PCR

 

5′-ATGGCTTCTCGCATCCTAT-3′(reverse)

 

HULC

5′-CCATCCAATCGGTAGTAGCG-3′(forward)

Real-time PCR

 

5′-TCCAGAAAGAGGGAGTTG-3′(reverse)

 

LET

5′-CCTTCCTGACAGCCAGTGTG-3′(forward)

Real-time PCR

 

5′-CAGAATGGAAATACTGGAGCAAG-3′ (reverse)

 

lncRNA-ATB

5′-TCTGGCTGAGGCTGGTTGAC-3′(forward)

Real-time PCR

 

5′-ATCTCTGGGTGCTGGTGAAGG-3′(reverse)

 

PVT-1

5′-GCTGCAAGGTCAAGATGGTT-3′(forward)

Real-time PCR

 

5′-GCTGGGTGGCGTTCTATC-3′(reverse)

 

β-actin

5′-TCCCTGGAGAAGAGCTACGA-3′(forward)

Real-time PCR

 

5′-AGCACTGTGTTGGCGTACAG-3′(reverse)

 

GADPH

5′-GCATCCTGGGCTACACTG-3′(forward)

Real-time PCR

 

5′-TGGTCGTTGAGGGCAAT-3′(reverse)

 

lncTCF7 promoter

5′-AGCCAGACAGAAGAGTGGA-3′ (forward)

ChIP-PCR

 

5′-TGGGATGGGGATGTCAGAAC-3′ (reverse)

 

lncTCF7 promoter SIE3

5′-ACTGGTACCTAAGCGGAGAGAGTCCCACACAGG-3′ (forward)

Site-directed mutagenesis

 

5′-ACTAAGCTTGAGTCAGAGTTCCCCAC-3′ (reverse)

 

lncTCF7 promoter SIE4

5′-ACTGGTACCTAAGCGGAGAGAGTCCCACACAGG-3′(forward)

Site-directed mutagenesis

 

5′-ACTAAGCTTGAGTCAGAGTTCCCCAC-3′ (reverse)

 

lncTCF7 siRNA-1

5′-AGCCAACATTGTTGGTTAT-3′,

RNA interference

lncTCF7 siRNA-2

5′-CACCTAGGTGCTCACTGAA-3′

RNA interference

STAT3 siRNA

5′-AAAUCCAGAACCCUCUGACAUUUGC-3′

RNA interference

siRNA control

5′-UUCUCCGAACGUGUCACGUTT-3′

RNA interference