Inhibition of oleandrin on the proliferation and invasion of osteosarcoma cells in vitro by suppressing Wnt/β-catenin signaling pathway

Table 1 The details of primers in semi-quantitative RT-PCR

Gene	Primer sequence (5’ to 3’)	Annealing temperature (°C)	Cycle number
c-Myc	F: CCACACATCAGCACAACTACG	57	30
	R: CCGCAACAAGTCCTCTTCAG	57	30
cyclin D1	F: TCGGGAGAGGATTAGGTTCC	57	30
	R: GTCACTGGATGGTTTGTTGG	57	30
survivin	F: GTCCCTGGCTCCTCTACTGTT	57	30
	R: GATGTGAAGGTTGGGCTGAC	57	30
MMP-2	F: GACCACAGCCAACTACGATG	59	30
	R: CACAGTCCGCCAAATGAAC	59	30
MMP-9	F: CATCGTCATCCAGTTTGGTGT	57	32
	R: AGGGTTTCCCATCAGCATT	57	32
β-actin	F: AGCGAGCATCCCCCAAAGTT	59	25
	R: GGGCACGAAGGCTCATCATT	59	25

ISSN: 1756-9966