Fig. 2
From: WT1-AS promotes cell apoptosis in hepatocellular carcinoma through down-regulating of WT1
Apoptosis promoting and proliferation inhibiting effect of WT1-AS by targeting WT1. a and b Expression of WT1-As and WT1 determined in various human HCC cell lines indicated in the figure. c Detection of WT1-AS and WT1 expression by real-time PCR after transfection (Upper panel). WT1 expression by western-blot in L02 and HepG2 cells treated with WT1-AS (lower panel). d and e Proliferation curve exampled by EdU assay for L02 and HepG2 cells treated with various conditions. f and g Cell apoptosis determined detected by PI-Annexin V staining assay for L02 and HepG2 cell treated with various condition indicated in the figure. All data presented as mean ± SD, *, P < 0.05, **, P < 0.01 by unpaired Student’s t test