Fig. 1

Anti-proliferative effects of D11 in human cancer cell lines. a M059K and MIA PaCa-2 cell lines were treated with increasing concentrations of D11 for a variable amount of time, respectively. Control experiment refers to cells treated with vehicle (0.1 % DMSO). The proportion of viable cells was determined by WST-1 assay and expressed in arbitrary units as a difference in absorbance measured at 450 nm and 690 nm (reference) wavelengths, respectively (mean +/− standard deviation, N = 6). Asterisks denote statistical significant differences between control and D11-treated cells for the corresponding time points, *, P <0.0001. b Flow cytometry analysis of cells treated with 0.1 % DMSO (Control) and increasing concentrations of D11 for 24 h (M059K) and 48 h (MIA PaCa-2), respectively. The amount of cells in the various phases of the cell cycle is indicated in percentage. Experiments were repeated three times obtaining similar results. Data from one representative experiment are shown