Fig. 6

D11 treatment results in impaired cell migration and decreased expression of NKCC1. a M059K and MIA PaCa-2 cells were treated with vehicle or D11 as indicated in the figure and subjected to wound-healing assay. D11 was added to the cells 4 h before the wounds were made. Unwounded areas were measured as described in the Materials and methods (N = 6, *, P <0.05, **, P <0.0001). Original magnification: 50x. b Cells were treated with D11 essentially as described in Fig. 4. Stimulation with IGF-1 was performed for 15 min. β-actin detection was carried out as loading control. Experiments were repeated three times obtaining similar results