Fig. 4
The regulation of rpS6 in cells migration and invasion. a Wound healing assays showed that the t-rpS6 and p-rpS6 overexpression in HBE (HBE + oe-rpS6) caused a much faster the healing recover than the two controls (×200). The expressions of N-cadherin, vimentin, MMP-2 and p-Paxillin were remarkably upregulated, but E-cadherin decreased significantly (All P < 0.05), in spite of the vague changes of Paxillin and MMP-9 (All P > 0.05). b Targeted knockdown of t-rpS6 and p-rpS6 (sh1-rpS6 and sh2-rpS6) notably inhibited the metastatic potentials of H1650 and SK-MES-1 cells (×400). The expressions of relative proteins, including E-cadherin, N-cadherin, vimentin, MMP-9, MMP-2 and p-Paxillin changed greatly as well (All P < 0.05). All experiments were carried out triplicate. Data are presented as mean ± SD. *: vs the corresponding blank control cell lines without any transfection (HBE, H1650 and SK-MES-1 respectively), P < 0.05; #: vs the corresponding control cell lines with the negative control (NC) vectors transfection (HBE + oe-NC, H1650 + sh-NC, SK-MES-1 + sh-NC, respectively), P < 0.05