Fig. 2
Quantification of human prostate cancer cells arriving and colonizing the bone marrow of male mice. a The DiD labelled tumour cells entering the bone microenvironment were visualized and quantified using two-photon microscopy on day 1, 7 and 21 post tumour cells injection. Scale bar = 200μm. b The number of tumour cells per mm3 bone marrow volume and c the minimum distance from tumour cells to the nearest bone surface was quantified and compared between 6-week old (Young) or 16-week old (Mature) mice using the Volocity 3D Image Analysis software. n > 6, * P < 0.05, ** P < 0.01, *** P < 0.001, t-test