Skip to main content
Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: The frequency of osteolytic bone metastasis is determined by conditions of the soil, not the number of seeds; evidence from in vivo models of breast and prostate cancer

Fig. 4

Comparison of skeletal metastases by human breast cancer cells in young and mature female mice. A single-cell suspension of 0.75 × 105 DiD labelled MDA-MB-231-luc2 cells/100 μL PBS was injected into the left cardiac ventricle of 6-week old (Young) or 16-week old (Mature) female BALB/c nude mice. a Tumour growth was monitored using the in vivo imaging system (IVIS). Skeletal tumours (red circled) were identified and confirmed by further anatomical examination post mortem up to 8 weeks post injection. b The bone lesions were examined and confirmed from 3D bone models created via micro-CT scanning tumour bearing femurs of young mice. c BV/TV was then quantified and compared between tumour bearing and non-tumour bearing legs of young mice, n = 4. d The number of skeletal tumours per mouse, e the number of non-skeletal tumour per mouse (skeletal muscle, liver, adrenal glands, but not peri- or intra-cardial tumours), and f the total tumour burden (radiance of luminescence) were also compared between 6- and 16-week old mice, n > 6. (* P < 0.05, ****P < 0.0001, t-test)

Back to article page