Fig. 5
Quantification of human breast cancer cells arriving and colonizing the bone marrow of female mice. a The DiD labelled tumour cells entering the bone microenvironment were visualized and quantified using a two-photon microscopy on day 1, 7 and 21 post tumour injection. Scale bar = 200μm. b The number of tumour cells per mm3 bone marrow volume and c the minimum distance from tumour cells to bone surfaces were quantified and compared between 6- and 16-week old mice using the Volocity 3D Image Analysis software. n > 6, ** P < 0.01, t-test