Fig. 1

Germline expression by qRT-PCR of the APC and MUTYH genes. The figure shows the results from qRT-PCR analysis conducted in 39 patients carrying either APC mutation, MUTYH mutation or none mutations in both genes, compared to 42 controls. mRNA isolated from PBMCs was quantified by qRT-PCR using GUSB gene as endogenous control. Data were analyzed using the comparative Ct method and are expressed as mean and standard error (SE) of relative expression, which corresponds to the 2⁻ΔCt value; *P < 0.05 versus control group with post hoc test. a Relative expression levels of APC gene and associated table showing 2⁻∆Ct mean values ± SE of each subgroup. b Relative expression levels of MUTYH gene and associated table showing 2⁻∆Ct mean values ± SE of each subgroup