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Fig. 8 | Journal of Experimental & Clinical Cancer Research

Fig. 8

From: HOXD9 promotes epithelial–mesenchymal transition and cancer metastasis by ZEB1 regulation in hepatocellular carcinoma

Fig. 8

HOXD9 binding to the promoter of ZEB1 were assayed by ChIP-qPCR. a Effect of silencing HOXD9 expression on the activity of the ZEB1 promoter in MHCC97H and its control cells. b Effect of ectopic HOXD9 expression on the activity of the ZEB1 promoter in Huh7 and its control cells. Data are presented as the ratio of firefly luciferase activity to sea pansy luciferase activity. pGL3 was used as a negative control for background. c Schematic of the ZEB1 promoter region. d and f qChIP was performed in HOXD9 silencing (d) and overexpression (f) cell lines. e and g IgG was used as a negative control. P < 0.01 is based on Student’s t-test. Error bars, SD

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