Fig. 1

ERα bound to the MTA1 promoter and downregulated MTA1 expression. a Schematic representation of the MTA1 promoter region with three half-ERE sites. Specific primers amplified –512 to −178 by PCR. b Chromatin immunoprecipitation (ChIP) assays used normal IgG or anti-ERα to identify ERα binding sites on the MTA1 promoter in HepG2 cells. PCR product was 335 base pairs. Three half-ERE sites were deleted from the wildtype MTA1 promoter for the mutant MTA1 promoter. Hep3B (c) and HepG2 (d) cells were cotransfected with luciferase vectors with wildtype or mutant MTA1 promoter or control vector. Results were from three independent experiments and presented as mean ± SEM. *P < 0.05 by t-test