Fig. 3

ERα suppressed proliferation and invasion of HCC cells. a CCK-8 assays showed that overexpression of ERα inhibited Hep3B cell growth. Absorbance at 450 nm was measured. Results were from three independent experiments and presented as mean ± SEM. b Bright cyan, EdU positive. EdU assays showed that ERα overexpression decreased the percent of EdU-positive in Hep3B cells compared to controls (n = 3, mean ± SEM). c Overexpression of ERα reduced invasion by Hep3B cells in transwell assays (n = 3, mean ± SEM). d CCK-8 assays showed that knockdown of ERα increased HepG2 cell growth (n = 3, mean ± SEM). e Bright cyan, EdU-positive. EdU assays showed that ERα knockdown increased the percent of EdU-positive HepG2 cells compared to controls (n = 3, mean ± SEM). f Knockdown of ERα increased invasion by HepG2 cells in transwell assays (n = 3, mean ± SEM) *P < 0.05 by t-test