Fig. 3

EGF/EGFR-mediated signals contribute to the migratory potential of osteosarcoma cells. Transwell migration assays (two experiments in duplicate) were performed for 24 h and 48 h in growth medium with 1 % FCS without and with addition of gefitinib as indicated. Migrated cells at the lower side of the membrane were counted microscopically in 4 optical fields/membrane in duplicate and normalized to the untreated control. One-way ANOVA with Bonferroni’s post hoc test; * p < 0.05; ** p < 0.01; *** p < 0.001