Fig. 2

RB family proteins and mRNA modulation in HuH-7 parental and HuH-7-CORE cell lines. a and b Western blot analysis of pRb, pRb2/p130 and p107 proteins in parental HuH-7 and in core expressing HuH-7-CORE cell lines, showing a marked down-modulation of pRb and the phosphorylated form of pRb2/p130 in the HuH-7-CORE cell line. c Quantitative PCR determination of mRNA levels relative to the three RB family gene products, as indicated, in the parental HuH-7 cell line (grey bars, which refer to a 100 % relative amount) and in the HuH-7-CORE cells (black bars) constitutively expressing HCV core protein. Statistical significance: *P = <0.05; **P = <0.01