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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: Peritumoural neutrophils negatively regulate adaptive immunity via the PD-L1/PD-1 signalling pathway in hepatocellular carcinoma

Fig. 3

PD-L1 expression was up-regulated on neutrophils in patients with HCC, and was negatively correlated with patients’ survival. a Representative PD-L1 expression on neutrophils and PD-1 expression on CD4+/CD8+ T cells from the blood of HD and HCC patients. b Tissue-infiltrated neutrophils from tumour tissue and peritumoural tissue were isolated and analyzed by FACS analysis. c Calculative percentage of PD-L1+ neutrophils, PD-1+ CD4 T cells and PD-1+ CD8 T cells in circulation of HD and HCC and in intra-tumoural and peri-tumoural tissue, by gating neutrophils, CD4+ T cells and CD8+ T cells. d Kaplan–Meier estimates of the RFS in patients with high (red line) and low (blue line) PD-L1+ neutrophil/PD-1+ T cell ratios in peritumoural (P < 0.0001, n = 64) and intratumoural tissues (P = 0.176, n = 65). HD, healthy donors

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