Fig. 1

AKAP4 gene and protein expression. a RT-PCR and qPCR analysis shows AKAP4 mRNA expression in various CRC cell lines along with normal colon (NC colon). b Western blot analysis demonstrates AKAP4 protein expression in various CRC cell lines and in normal colon cells (NC colon). c Flow cytometric analysis reveals AKAP4 expression in COLO 205 and HCT 116. d Indirect immunofluorescence and co-localization studies show the cytoplasmic localization and co-localization (orange-yellowish staining) of AKAP4 in endoplasmic reticulum, Golgi bodies and mitochondria in COLO 205 and HCT 116 CRC cells, however no co-localization was observed in nuclear envelope