Fig. 3
From: A-kinase anchor protein 4 (AKAP4) a promising therapeutic target of colorectal cancer
AKAP4 gene and protein silencing initiates apoptosis. a The representative scanning electron microscope (SEM) images show changes in phenotypic characteristics of COLO 205 and HCT 116 cells when transfected with AKAP4 shRNA3 as compared to NC shRNA. AKAP4 shRNA3 treatment shows intense membrane blebbing and holes on the surface of cells at 24 h and 48 h as compared to cells when treated with NC shRNA. b The blots show changes in the expression of various molecules that are involved in apoptotic pathway in COLO 205 and HCT 116 cells when transfected with AKAP4 shRNA3 as compared to NC shRNA. The results demonstrates up regulation of pro-apoptotic molecules like AIF, APAF1, BAD, BID, BAK, BAX, PARP1, PUMA, NOXA, cyt-C and Caspase 3, Caspase 7, Caspase 8 and Caspase 9, while down regulation of anti-apoptotic molecules like Bcl-xL, cIAP2, XIAP, BCL2, Survivin, and Axin 2. β-actin was used as a loading control