Fig. 5
From: A-kinase anchor protein 4 (AKAP4) a promising therapeutic target of colorectal cancer
Ablation of AKAP4 protein inhibits tumor growth. a Graph depicts the significant difference in the tumor volume when treated with AKAP4 shRNA3 as compared to NC shRNA. b Histogram shows the significant reduction in tumor weight of animals treated with AKAP4 shRNA3 as compared to NC shRNA. c Representative images of tumor excised from the animals treated with AKAP4 shRNA3 as compared to NC shRNA. d Western blot analysis shows reduced expression of AKAP4 and PCNA in AKAP4 shRNA3 treated mice as compared to NC shRNA treated mice. β-actin was used as a loading control. e Immunohistochemical analysis on the serial sections of tumor reveals reduced expression of AKAP4 (middle panel) and PCNA (right panel) in AKAP4 shRNA3 treated mice as compared to NC shRNA treated mice. The left panel shows the cytostructure of tumour by H&E staining. f Histograph depicts significant reduction in AKAP4 and PCNA expression in AKAP4 shRNA3 treated mice as compared to NC shRNA treated mice. **P < 0.001, ***P < 0.0001. Original magnification x200, objective x20