Fig. 6
From: A-kinase anchor protein 4 (AKAP4) a promising therapeutic target of colorectal cancer
Immunohistochemical analysis of various molecules involved in cell cycle, apoptosis and EMT. a Representative micrographs depicts reduced immuno-reactivity of CDK1, CDK2, CDK4, CDK6, Cyclin D1, Cyclin E,Cyclin B1, and increased immuno-reactivity of p16 and p21 in AKAP4 shRNA3 treated mice as compared to NC shRNA treated mice. b Representative photomicrographs show increased immuno-reactivity of AIF, APAF1, BAD, BID, BAK, BAX, PARP1, PUMA, NOXA, cyt-C in AKAP4 shRNA3 treated mice as compared to NC shRNA treated mice. c Representative photomicrographs show increased immuno-reactivity of Caspase 3, Caspase 7, Caspase 8 and Caspase 9 and decreased immuno-reactivity of BCL2, Bcl-xL, cIAP2, XIAP, Survivin in AKAP4 shRNA3 treated mice as compared to NC shRNA treated mice. d Representative micrographs showing increased immuno-reactivity of E-cadherin and reduced immuno-reactivity of N-cadherin, α-SMA, SNAIL, TWIST, Vimentin, MMP2, MMP9 in AKAP4 shRNA3 treated mice as compared to NC shRNA treated mice. Original magnification x200, objective x20