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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: Phenotype plasticity rather than repopulation from CD90/CK14+ cancer stem cells leads to cisplatin resistance of urothelial carcinoma cell lines

Fig. 5

Long-term cisplatin treated UCCs are not enriched for CD90+/CK14+ cells. a Relative cell number in RT-112-LTT and J82-LTT and parental cell lines was measured by MTT after 24, 48, 72 and 96 h. Population doubling time was calculated based on raw absorbance data. b Clonogenic potential of RT-112-LTT and J82-LTT and parental cell lines without or with cisplatin, Giemsa staining. c Mean percentages of CD90+ cells in untreated (dark grey bars) and LTT (dark grey bars, shades), CD44+ cells in untreated (light grey bars) and LTT (light grey bars, shaded), and CD49f+ cells in untreated (grey bars) and LTT (grey bars, shaded) UCCs as measured by flow cytometry. d Relative expression of CK7, CK13, and CK14 in untreated and LTT-UCCs. Expression levels in the untreated control were set as 1. SDHA was used as a reference gene and relative expression was calculated by the 2−ΔΔCT method. Values represent the mean ± SD of biological triplicates. Untr Ctrl untreated control, LTT long-term cisplatin treatment, PDT population doubling time, na not available. *P <0.05; **P <0.001

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