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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: LDOC1 inhibits proliferation and promotes apoptosis by repressing NF-κB activation in papillary thyroid carcinoma

Fig. 4

Influence of LDOC1 overexpression on TPC-1 cell growth and apoptosis. a TPC-1 cell numbers: UNT cells and cells transduced with Lv-LDOC1 or Lv-NC; cell numbers at the indicated times were estimated using CCK-8 proliferation assays. Compared with UNT cells, TPC-1 cells transduced with Lv-LDOC1 showed a significant reduction in cell number starting from Day 3. b LDOC1 restoration induces an increment of cells in subG1 phase. TPC-1 cells transduced with Lv-NC or Lv-LDOC1 were seeded into 6-well plates and cultured overnight and then serum-starved for 24 h; next, the culture medium was replaced with medium containing FBS, and after culturing for another 24 h, the cells were trypsinized and cell cycle distribution was analyzed by performing propidium iodide staining and flow cytometry. c Apoptosis assessment in TPC-1 cells. Cells transduced as in (b) were collected and stained using an Annexin V-APC/7-AAD apoptosis-detection kit. The upper and lower right quadrants display the percentages of apoptotic cells. d Levels of Bax, Bcl-2, and Bcl-xL determined using qRT-PCR. e Western blot analyses of Bax, Bcl-2, Bcl-xL, caspase-3, and PARP; β-actin was used as a loading control. *P < 0.05, **P < 0.01, and ***P < 0.001

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