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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: LDOC1 inhibits proliferation and promotes apoptosis by repressing NF-κB activation in papillary thyroid carcinoma

Fig. 5

LDOC1 inhibits NF-κB activation in TPC-1 cells. a Immunoblotting analysis of p65 expression in the nucleus and the cytoplasm. TPC-1 cells transduced with Lv-LDOC1 or Lv-NC were stimulated with TNFα (10 ng/mL) for 30 min. Proteins from nuclear and cytoplasmic fractions were extracted and immunoblotted; histone H2A and β-actin were used as loading controls. b Stable LDOC1 overexpression inhibited basic and TNFα-stimulated NF-κB activation. Cells transduced as in (a) were transiently transfected with an NF-κB-luciferase expression vector. At 24 h after transfection, cells were serum-starved for a further 24 h, after which they were stimulated with TNFα (10 ng/mL) for 24 h and used in luciferase activity assays. Results are presented as luciferase activity values relative to the value in cells transfected with the control vector. c Western blot analysis of IκBα, c-Myc, Bax, and Bcl-xL in Lv-NC and Lv-LDOC1 cells. Cells were treated with TNFα (10 ng/mL) for 30 min or 24 h (IκBα), and tubulin was used for normalization. All data (a-c) are representative of three independent experiments. d Viability of Lv-LDOC1 and Lv-NC cells was determined using a CCK-8 proliferation assay. Cells were stimulated with the indicated concentrations of TNFα once every 2 days together with fresh medium for 5 days. The data shown represent three independent experiments performed in triplicate

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