Fig. 6

LDOC1 restores the responsiveness of TPC-1 cells to TGF-β1 inhibitory signaling. a TPC-1 cells transduced with Lv-NC or Lv-LDOC1 were treated with or without rTGF-β1 (5 ng/mL) for the indicated times. The levels of nuclear p65 and IκBα were determined by performing western blot analysis, with histone H2A and β-actin being used as loading controls, respectively. b, c Cell cycle arrest and cell viability were assessed in Lv-NC or Lv-LDOC1 cells treated as in (a). *P < 0.05, **P < 0.01, and ***P < 0.001 compared with Lv-NC cells (Student’s t test). All data are presented as means ± SEM from three independent experiments