Fig. 5

Effect of vernodalin on modulation of PI3K/Akt signalling. MCF-7 cells and MDA-MB231 were left untreated or treated with vernodalin at indicated concentration for 24 h (a & b) Expression of Total Akt, p-Akt, Total PI3K and p-PI3K were analysed by immunoblot analysis. Respective blots were quantified using Image J software (c & d). E & F shows the ratio between P-PI3K and total PI3K protein. All the treatment groups were compared with control. “*” denotes statistically significant at P < 0.05. Results are shown as percentage values ± SD of three independent experiments