Fig. 2

MTA1 enhances the invasion and migration in MPM cells. a and b MTA1 level of MPM wild-type cells (MSTO-211H and H2452) and MPM cells transfected by lentivirus containing pLL3.7-empty and pLL3.7-shMTA1 by real-time PCR. c MTA1 expression of MPM wild-type cells (MSTO-211H and H2452) and MPM cells transfected by lentivirus containing pLL3.7-empty and pLL3.7-shMTA1 by western-blot. d and e The result of transwell assay showed that the invasion ability of MSTO-211H was significantly suppressed after MTA1 silencing, which was confirmed by integrated optical density (IOD) value evaluation. f and g Similar effect of MTA1 was observed in H2452 MPM cells. h and i The result of wound-healing assay showed that the migration ability of MPM cells was restrained after MTA1 silencing at 48 h time point. Data are represented as mean ± SD. *p < 0.05, **p < 0.001. j and k, The viability of MPM wild-type cells (MSTO-211H and H2452) and MPM cells transfected by lentivirus containing pLL3.7-shMTA1 were assessed by CCK-8 assay at 0 h, 24 h, 36 h and 48 h time point. MTA1, metastasis-associated gene 1; MPM, malignant pleural mesothelioma; PCR, polymerase chain reaction; CCK-8, cell-counting kit 8