Fig. 3

MTA1 suppressed the expression of E-cadherin and was up-regulated by TGF-β1 inducing. a and b Nine metastasis genes varied larger than two folds were picked via PCR array and were verified by real-time PCR in MPM cells. c Western-blot assay validated the result of PCR array of negative trend of MTA1 and E-cadherin in MPM cells. d and e Luciferase report assay demonstrated that E-cadherin transcription was gradually enhanced in MPM cells transfected by lentivirus containing pLL3.7-shMTA1 in a time-dependent manner. f MTA1 mRNA expression was up-regulated in TGF-β1-stimulated MPM cells. g MPM patients with a high expression level of MTA1 had a significantly shorter overall survival compared with patients with a low expression level of MTA1. Data are represented as mean ± SD. *p < 0.05, **p < 0.001. MTA1, metastasis-associated gene 1; MPM, malignant pleural mesothelioma; TGF-β1, transforming growth factor beta 1; PCR, polymerase chain reaction