Fig. 1

PRX1 silencing enhances cancer cell sensitivity to vitK3. a–d MTT assay on HeLa (a), A549 (b), HUVEC (c) cells and primary fibroblasts (d) transiently transfected with PRX1 specific siRNA or control siRNA for 48 h followed by vitK3 treatment with indicated doses for 4 and 24 h. The inserts are western blot to verify efficiency of PRX1 knockdown in siRNA transfected cells. e MTT assay on HeLa cells with stable PRX1 knockdown (Prx1–) and non-silenced control cells (Prx1+) treated with indicated doses of vitK3 for 4 and 24 h. f Clonogenic assay for Prx1+ and Prx1– cells treated with indicated doses of vitK3 for 4 h then recovered for 10 days. Representative scanned images of 6-well plates are presented (left panel). Clonogenic ratio = (colony number in vitK3-treated sample / colony number in DMSO-treated control sample) (right panel). Data are mean ± SD of at least three independent experiments. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001