Fig. 3

VitK3-induced cytotoxicity in Prx1– cells involves necroptosis-like death. a Detection of MMP by TMRM in cells exposed to vitK3 at indicated time points. One set of three independent results is presented. b Time course of annexin V/PI double staining in cells exposed to 15 μM vitK3. One representative set of dot plots is presented to show distribution of different cell populations at indicated time points. Percentage of each population is indicated. c Detection of PARP1 cleavage and PAR activation by western blot in the presence of indicated concentrations of vitK3 for defined times