Fig. 1

Construction and rescue of rNDV-18HL. a Schematic diagram of the rNDV-18HL genome which contained the genes of light and heavy chains of human-mouse chimeric antibody cHAb18. Two transcriptional cassettes containing light and heavy chains with gene start (GS) and gene end (GE) signals were inserted into the AgeI (isoaudamers of Kpn2I) site between F and HN genes. b Syncytia cells induced by recombinant rNDV-18HL which was rescued by co-transfection of pBR-rNDV-18HL with three helper plasmids into BSR-T7/5 cells. BSR-T7/5 cells co-transfected with three helper plasmids were served as control. Scale bar = 200 μm