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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: Oncolytic Newcastle disease virus expressing chimeric antibody enhanced anti-tumor efficacy in orthotopic hepatoma-bearing mice

Fig. 2

Identification of rNDV-18HL. a BHK-21 cells were infected with rNDV-18HL and the human IgG was purified from the supernatant for detecting cHAb18 expression using western blot analysis. The purified cHAb18 antibody from stably transfected CHO clone was served as control. b The level of cHAb18 antibody was determined by ELISA in culture supernatants of rNDV-18HL-infected BHK-21 cells at different time of post-infection (n = 5). c One-shot kinetics for the interaction between virally expressed cHAb18 antibody and CD147 antigen was detected with surface plasmon resonance technology. Each set of five sensograms displayed the responses to the five diluted concentrations of recombinant CD147 interacting with one immobilization level of cHAb18 antibody. RU was denoted as resonance unit

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