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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: Oncolytic Newcastle disease virus expressing chimeric antibody enhanced anti-tumor efficacy in orthotopic hepatoma-bearing mice

Fig. 4

The inhibition of HCC cell motility by virally expressed cHAb18 and rNDV-18HL. a Wound healing assay detected the migration of SMMC-7721 cells which were treated with virally expressed cHAb18 for 24 h. b The relative wound closure was measured using ImageJ (n = 5). Scale bar = 500 μm. c SMMC-7721 cells in transwell chamber coated with matrigel were treated with virally expressed cHAb18 for 24 h. The invaded cells were stained with crystal violet. Scale bar = 200 μm. d The invaded cells were quantified by determining the absorption at 570 nm (n = 3). e Transwell migration assay detected the migration of SMMC-7721 cells which were treated with either rNDV-18HL or NDV Italien. The cells on the lower side of membrane were stained with crystal violet. Scale bar = 200 μm. f The stained area of each image was measured using ImageJ (n = 5). * P < 0.05, ** P < 0.01, *** P < 0.001

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