Fig. 1

Characterisation of nanocapsules in the different experimental conditions. a Atomic Force Microscopy (AFM) images of height (left) and phase (right) showing the Differences in The PUFA-loaded NC size dispersion and Morphology (scan Sizes are 10×10 μm). b Fractograms Of the different PUFA nanocapsules After Asymmetrical-Flow-Field-Flow-Fractionation (AsF4). Black Dots represent Detection by DLS (Z-average hydrodynamic diameter) and the blue trace represents UV detection. Green dots (DLS) and red full line (UV-intensities) refer to unloaded PεCL NC for comparison in each graph. The inserts represent the traces of 92o angle analysis from peak selection zones (brown bars) where the MALLS Analysis was performed to provide radius of gyration (Rg, Root mean square radius) (red Line and left Y axis) and detector signal intensity in volume (blue Line and right Y axis). The First peak at short retention times is the void volume