Fig. 4

Effect of free or encapsulated 4(RS)-4-F4t-neuroprostane on the growth the breast carcinoma cell line MDA-MB-231. Cells were exposed 4 days to 0.3, 1, 3, 10, 30, 50 and 100 μM then cell proliferation was measured by the MTT method (see Materials and methods). The relative cell proliferation (absorbance of test wells) was expressed as a percentage of the control (line 100 %) that was not treated with 4(RS)-4-F4t-neuroprostane (absorbance of control wells with vehicle or NC with vehicle). * Significantly different between the free and encapsulated substance at the same concentration, at P < 0.05. Data points are mean ± SEM of three separate experiments, each one performed in three wells