Fig. 2
Effect of TMZ and RGs on cell cycle and apoptosis of HUVECs. a Representative flow cytometry histograms of cell cycles. Treatment with TMZ (80 μg/ml) or RG3 (80 μg/ml) for 72 h led to accumulation of S phase and appearance of sub-G0 apoptotic cells. b HUVEC cell cycle arrested at S phase after treatment with TMZ. c. HUVEC cell cycle arrested at S phase after treatment with RG3. d Rates of apoptotic cells after treatment with TMZ. e Rates of apoptotic cells after treatment with RG3. f Additive effect of TMZ and RG3 on cell apoptosis. g A representative image of DNA ladder after treatment with TMZ (80 μg/ml) or RG3 (80 μg/ml) for 48 h. h Realtime PCR data showed that TMZ and RG3 additively reduced both VEGF and Bcl-2 expression compared to vehicle treated control, which was set to 100 %. Bars with different letters indicate significant difference (P < 0.05) based on post-hoc Tukey analysis