Fig. 1
PGCCs with budding daughter cells in MCF-7 and MDA-MB-231 cells. A. MCF-7 PGCCs and control MCF-7 cells. a. Control MCF-7 cells (×400). b. MCF-7 PGCCs induced by 450 μM CoCl2 treatment for 72 h (×400). Small black arrowheads indicate budded daughter cells; large black arrow heads indicate PGCCs. c. PGCCs generated daughter cells via budding 10–15 days after CoCl2 treatment. Black arrowheads indicate budded daughter cells (×100). d. Fast reproduction of PGCCs by generated daughter cells via budding (×100). B. MDA-MB-231 PGCCs and control MDA-MB-231 cells. a. Control MDA-MB-231 cells (×400). b. MDA-MB-231 PGCCs induced by 300 μM CoCl2 treatment for 72 h (×400). Small black arrowheads indicate budded daughter cells; large black arrowheads indicate PGCCs. c. PGCCs generated daughter cells via budding 10–15 days after CoCl2 treatment. Small black arrowheads indicate budded daughter cells; large black arrowheads indicate PGCCs (×100). d. Recovery of PGCCs by generated budding daughter cells (×100)