Fig. 6

Inhibitor of MEK reduces Gab2-induced EMT, cell migration and invasion. a SW480-Gab2 cells were treated or not with 20 μM U0126 during 16 h after which proteins were analyzed by western blot with specific antibodies against E-cadherin, vimentin, MMP7, MMP9, phosphorylated ERK1/2 and total ERK1/2. b and c SW480-Gab2 cells were subjected to transwell migration and invasion assays in the absence (vehicle), or presence of 20 μM U0126. ***P < 0.001