Fig. 6

The establishment and characterization of tumor-associated macrophages. a The PMA-treated THP-1 cells were differentiated into adherent macrophages. b The Double immunofluorescence staining of CD68 (red) and CD163 (green) in the TAMs. The nuclei were stained with DAPI. The photographs were taken at 200x magnification. c The inflammatory cytokines and chemokines were detected in conditioned medium by using mass spectral analysis